Production and characterization of crude proteases from Halobacillus salinus strain DZ28 newly isolated from salt lake sediments in Algeria and their use as detergent bioadditives

New Algerian Gram-positive, rod-shaped, endospore-forming, salt-philic bacteria (DZ28 strain) that overproduce extracellular alkaline proteases have been isolated from salt lake deposits in Lake Oubeira, El Taref. Strain DZ28 was assigned as Halobacillus salinus on the basis of phenotypic properties and 16S rDNA gene sequencing (ripotyping). The maximum protease activity registered after 36 hours incubation optimized medium at 30 ° C 19,000 U / ml a shaking bottle culture 160 rpm. crude extract showed optimal 60 temperature pH 12. It is actively inhibited by PMSF DIFP, indicating it belongs to serine family. Interestingly, not only very stable nonionic surfactants oxidants, but also high stability compatibility with some commercial detergents. retaining more than 100% its initial pre-incubation for 1 h 40°C ISIS, followed Pril (98%), Tide (95%) Dixan (90%). More curiously, wash overall performance evaluation discovered may dispose blood-stains remove low supplementation (500 U/mL). This first report has potential promising candidate future applications bioadditive detergent formulations.